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M9460573.TXT
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1994-06-25
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Document 0573
DOCN M9460573
TI HIV-2 and SIV infection of nonprimate cell lines expressing human CD4:
restrictions to replication at distinct stages.
DT 9408
AU McKnight A; Clapham PR; Weiss RA; Chester Beatty Laboratories, Institute
of Cancer Research,; London, United Kingdom.
SO Virology. 1994 May 15;201(1):8-18. Unique Identifier : AIDSLINE
MED/94233779
AB Mouse, rat, rabbit, cat, and mink cell lines expressing human CD4
restrict entry of HIV-1 strains at the cell surface. We now report that
CD4+ cat and rabbit cell lines are permissive to cell fusion induced by
several HIV-2 and SIV strains. CD4+ rat and mouse cells are only
sensitive to HIV-2 and SIV fusion in the presence of active amphotericin
B. HIV-2 and SIV replicate in CD4+ cat and CD4+ mink cells, but not in
the CD4+ rabbit, rat, or mouse cells. These latter cell lines poorly
express a transfected DNA molecular clone of HIV-2 DNA. CD4+ rat cells
are sensitive to entry by HIV-2 (HTLV-1) pseudotypes and also to HIV-2
if amphotericin B is present. Early reverse transcription then occurs
without subsequent viral gene expression, but the fusion-sensitive CD4+
rabbit cells are not permissive for HIV-2 reverse transcription. Our
observations demonstrate (i) that the cat and mink cell lines express
everything required except human CD4 for entry and replication by
several HIV-2 and SIV strains and (ii) that distinct postpenetration
blocks to viral replication are evident in rabbit and rodent cell lines.
DE Amphotericin B/PHARMACOLOGY Animal Antigens,
CD4/PHARMACOLOGY/*PHYSIOLOGY Base Sequence Cats Cell Fusion/DRUG
EFFECTS Cell Line, Transformed DNA Replication DNA, Viral/ANALYSIS
Human HIV Antigens/ANALYSIS HIV-2/*PHYSIOLOGY HTLV-I
Antigens/PHYSIOLOGY Mice Mink Molecular Sequence Data Rabbits Rats
Recombinant Proteins/PHARMACOLOGY Species Specificity Support,
Non-U.S. Gov't SIV/*PHYSIOLOGY Transfection Virus
Replication/*PHYSIOLOGY JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).